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Quantifoil R2/4 Holey Carbon Films - Circular

Quantifoil R2/4 Holey Carbon Films - Circular

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Product Details:

  • Melting Point Not applicable (carbon film)
  • Shape Circular
  • Poisonous Yes
  • Density 2.2 Gram per cubic centimeter(g/cm3)
  • Ph Level Neutral (pH 7)
  • Storage Store in dry, dust-free container at room temperature
  • Molecular Weight 12.01 g/mol
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Quantifoil R2/4 Holey Carbon Films - Circular Product Specifications

  • Film supported on grid
  • 12.01 g/mol
  • Transmission Electron Microscopy (TEM) sample support
  • 39219090
  • Quantifoil R2/4
  • Not applicable (carbon film)
  • 2.2 Gram per cubic centimeter(g/cm3)
  • Yes
  • Circular
  • Store in dry, dust-free container at room temperature
  • Neutral (pH 7)
  • Cryo-EM, specimen preparation, structural biology, electron microscopy
  • Odorless
  • 7440-44-0 (carbon)
  • Manufacturers specification
  • Holey carbon film, circular
  • Laboratory/Research Grade
  • C (amorphous)
  • >99% carbon
  • Microscopy consumable
  • C
  • Pure amorphous carbon on copper grid
  • Insoluble in water and most solvents
  • Amorphous carbon

Quantifoil R2/4 Holey Carbon Films - Circular Trade Information

  • Telegraphic Transfer (T/T), Paypal, Cash in Advance (CID), Cheque
  • All India

Product Description

Quantifoil R2/4 Holey Carbon Films - Circular 

Quantifoils®, produced by semiconductor lithographic techniques, are 15 - 20nm thick perforated carbon films with precise, well defined hole sizes, shapes and arrays. Quantifoil support films provide a high percentage of open area in addition to minimising total specimen thickness. Object distortions arising from interactions with the support film are also reduced. They are particularly suited for cryo applications, tomography and low dose microscopy (LEEP).

Circular hole, size 2µm dia, separation 4µm

Quantifoil type: R2/4

Quantifoils are supplied as 10 grids or 100 grids in a grid box.



Exceptional Support for TEM and Cryo-EM

Quantifoil R2/4 Holey Carbon Films offer a robust and stable base for imaging specimens at high resolutions. Their regular hole array optimizes sample distribution and preserves specimen integrity during microscopy, making them ideal for structural biology and advanced TEM research.


Optimized Hydrophilicity for Improved Adsorption

Post glow discharge, the carbon film surface becomes distinctly hydrophilic. This enhanced surface property increases specimen adherence and spreading, supporting clearer imaging and consistent sample presentation necessary for high-precision electron microscopy workflows.

FAQs of Quantifoil R2/4 Holey Carbon Films - Circular:


Q: How should Quantifoil R2/4 Holey Carbon Films be stored to maintain their stability?

A: It is recommended to store the films in a dry, dust-free container at room temperature. Doing so preserves their clean surface and structural integrity, supporting a shelf life of over one year under these conditions.

Q: What is the benefit of the 2 m hole size and 4 m spacing in these carbon films?

A: The specific hole size and regular spacing enable optimal specimen distribution and uniform support during TEM or cryo-EM imaging, resulting in lower background noise and improved visualization of biomolecules.

Q: When is it necessary to perform glow discharge on the Quantifoil films?

A: Glow discharge should be performed just before specimen preparation to render the carbon surface hydrophilic, enhancing sample adsorption and promoting even spreading of aqueous solutions used in electron microscopy.

Q: Where are Quantifoil R2/4 Holey Carbon Films typically utilized?

A: These films are primarily used in research laboratories for structural biology, specimen preparation, and imaging in cryo-Electron Microscopy (cryo-EM) and Transmission Electron Microscopy (TEM).

Q: What compatibility do these films offer for different sample preparation protocols?

A: Quantifoil R2/4 films are compatible with most standard cryo-EM sample preparation techniques due to their stable structure, optimal hole pattern, and hydrophilic surface, making them versatile across various microscopy workflows.

Q: How does electron beam evaporation contribute to the quality of these films?

A: Electron beam evaporation produces a uniform, pure amorphous carbon film, ensuring film thickness consistency and reproducible imaging performance across different batches.

Q: What are the advantages of using films with >99% purity amorphous carbon?

A: High-purity amorphous carbon minimizes contaminant interference, which helps achieve clear, high-resolution images and reliable results in sensitive research applications.

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